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Ribobio co
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Bioneer Corporation
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OriGene
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Spry1 Rat 3 unique 27mer siRNA duplexes 2 nmol each
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Image Search Results
Journal: IBRO Neuroscience Reports
Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma
doi: 10.1016/j.ibneur.2022.07.003
Figure Lengend Snippet: SPRY1 is highly expressed in GBM, A. SPRY1 expression in GBM was compared using RNA-seq of REMBRANDT dataset. Data are mean ± SEM (nontumor, n = 28; astrocytoma, n = 147; oligodendroglioma, n = 67; GBM, n = 219). * **P < .001. B. SPRY1 expression compared GBM grades (Grade I and II, n = 100; Grade III, n = 85; Grade IV, n = 130). * **P < .001. C. Using microarray of REMBRANDT dataset, Kaplan-Meier survival curves of 397 GBM patients (SPRY1 high n = 185, SPRY1 low n = 212, P < .0001). D. SPRY1 expression compared IDH-mutant GBM.
Article Snippet: Small interfering RNAs (siRNA) for inhibiting the
Techniques: Expressing, RNA Sequencing, Microarray, Mutagenesis
Journal: IBRO Neuroscience Reports
Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma
doi: 10.1016/j.ibneur.2022.07.003
Figure Lengend Snippet: SPRY1 is highly expressed in glioma cells and glioma stem cells than in normal astrocytes. A. Real-time qPCR analysis of SPRY1 mRNA expression in normal human astrocyte (NHA), glioma cells, and glioma stem cells. NHA vs. glioma cells or glioma stem cells. B and C. mRNA expression of SPRY1 , stem cell markers, and differentiation markers in NBE cultured and FBS cultured GSC11 (B) and GSC23(C). NBE vs. FBS in GSC11 and GSC23. Data are means ± SEM (n = 3). Data are means ± SEM (n = 3). *P < .05, * **P < .001, * ** *P < .0001.
Article Snippet: Small interfering RNAs (siRNA) for inhibiting the
Techniques: Expressing, Cell Culture
Journal: IBRO Neuroscience Reports
Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma
doi: 10.1016/j.ibneur.2022.07.003
Figure Lengend Snippet: Knockdown of SPRY1 inhibits the stemness of GSCs, A . Realtime-qPCR indicated SPRY1 knockdown efficiency using siRNA transfection in GSCs. B. Measurement of cell proliferation rate in NT (Non-targeting siRNA) and siSPRY1. *P < .05, * *P < .01. C. Effect of SPRY1 knockdown on neurosphere formation in GSC11. Data are means ± SEM (n = 3). * *P < .01. Scale bars represent 100 µm. D. Effect of SPRY1 knockdown on stem cell markers, CD15 and CD133 mRNA expression in GSC11. Data are means ± SEM (n = 3). *P < .05, * ** *P < .0001.
Article Snippet: Small interfering RNAs (siRNA) for inhibiting the
Techniques: Knockdown, Transfection, Expressing
Journal: IBRO Neuroscience Reports
Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma
doi: 10.1016/j.ibneur.2022.07.003
Figure Lengend Snippet: SPRY1 positively correlates with genes associated with cancer stem cell stemness pathway. A. Upregulated genes correlated with high expression of SPRY1 analysis to Gene Ontology analysis (GO analysis) in REMBRANDT dataset. B. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in REMBRANDT microarray dataset. C. Gene Set Enrichment Analysis (GSEA) in REMBRANDT; Representative enriched pathways in SPRY1 positive correlated samples.
Article Snippet: Small interfering RNAs (siRNA) for inhibiting the
Techniques: Expressing, Microarray
Journal: IBRO Neuroscience Reports
Article Title: Sprouty 1 is associated with stemness and cancer progression in glioblastoma
doi: 10.1016/j.ibneur.2022.07.003
Figure Lengend Snippet: SPRY1 correlated GBM subtype and region. A. Comparison of SPRY1 mRNA levels among the groups with GBM subtypes (Classical n = 99, Neural n = 39, Mesenchymal n = 37, Proneural n = 44) * ** P < .001. B. The graph of SPRY1 between GBM subtypes markers correlation in REMRANDT dataset. C. GSEA analysis of REMBRANDT dataset, SPRY1 positive samples correlated with GBM subtypes. D. Heatmap of SPRY1 expression signature correlated with GBM region in the Ivy GAP RNAseq dataset. CT, cellular tumor; IT, infiltration tumor; LE, leading edge; MVP, microvascular proliferation; HBV, hyperplastic blood vessels; PAN, pseudopalisading cells around necrosis; PNZ, perinecrotic zone.
Article Snippet: Small interfering RNAs (siRNA) for inhibiting the
Techniques: Comparison, Expressing